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Author | Edited by Ashok Agarwal , Ralf Henkel , Ahmad Majzoub |
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Table Of Content | Introduction R. John Aitken 1. Standard semen analysis: manual semen analysis Lars Björndahl 2. Standard semen analysis: computer-assisted semen analysis Liana Maree 3. Standard semen analysis: home sperm testing Manesh Panner Selvam Kumar, Christopher Douglas and Ashok Agarwal 4. Standard semen analysis: leukocytospermia Renata Finelli and Ralf Henkel 5. Standard semen analysis: morphology Roelof Menkveld 6. Sperm vitality: eosin-nigrosin dye exclusion Rakesh Sharma and Ashok Agarwal 7. Sperm vitality: hypo-osmotic swelling test Alexandre Rouen and Rajasingam S. Jeyendran 8. Determination of mitochondrial membrane potential by flow cytometry in human sperm cells Nicolas Germain, Nathalie Jouy, Carole Marchetti and Philippe Marchetti 9. Capacitation and acrosome reaction: fluorescence techniques to determine acrosome reaction Raúl S. Sánchez Gutiérrez, Fabiola Zambrano and Pamela Uribe 10. Capacitation and acrosome reaction: histochemical techniques to determine acrosome reaction Brett Nixon, Shenae Café, Elizabeth Bromfield, Geoffry De Iuliis and Matthew Dun 11. Zona binding: competitive sperm-binding assay De Yi Liu, Ying Zhong and Zi-Na Wen 12. Zona binding: hemizona assay Shubhadeep Roychoudhury and Daniel Franken 13. Oolemma binding: sperm penetration assay Anup Shah and Kathleen Hwang 14. Oxidative stress testing: direct tests Renata Finelli, Manesh Kumar, Panner Selvam and Ashok Agarwal 15. Oxidative stress testing: indirect tests Rakesh Sharma, Kathy Robert and Ashok Agarwal 16. Chromatin condensation: aniline blue stain Jesse JP, Vidhu Dhawan and Rima Dada 17. Chromatin condensation: chromomycin A3 (CMA3) stain Sara Marchiani, Lara Tamburrino, Francesca Benini, Sandra Pellegrini and Elisabetta Baldi 18. Sperm chromatin structure: Toluidine Blue Staining Mohammed Nasr-Esfahani and Marziyeh Tavalaee 19. DNA damage: TdT-mediated dUTP nick-end-labelling (TUNEL) assay Rakesh Sharma, Concetta Iovine and Ashok Agarwal 20. DNA damage: sperm chromatin structure assay (SCSA) Don Evenson 21. DNA damage: COMET assay Luke Simon, Benjamin R. Emery and Douglas T. Carrell 22. DNA damage: halo sperm test Jaime Gosálvez and José LuÃs Fernández 23. DNA damage: fluorescent in-situ hybridization (FISH) Sezgin GüneÅŸ 24. Clinical value of sperm function tests Ahmad Majzoub 25. Future developments: sperm proteomics Manesh Panner Selvam Kumar, Saradha Baskaran and Ashok Agarwal 26. Conclusion Ahmad Majzoub, Ralf Henkel and Ashok Agarwal. |
Publish Date | 30 Apr 2021 |